tissue microarrays for ihc breast cancer

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A ‘Waterfall’ Transfer-based Workflow for Improved Quality ...- tissue microarrays for ihc breast cancer ,Mar 07, 2014·A major focus in cancer research is the identification of biomarkers for early diagnosis, therapy prediction and prognosis. Hereby, validation of target proteins on clinical samples is of high importance. Tissue microarrays (TMAs) represent an essential advancement for high-throughput analysis by assembling large numbers of tissue cores with high efficacy and comparability. However ...Tissue microarray technology for high-throughput molecular ...Apr 01, 2001·Breast cancer 328 IHC ER, PR Methodology, comparison with whole sections 2000 (7) Breast cancer 380 IHC ER, PR, HER2 Methodology comparison 2000 (8) Breast cancer 113 IHC CCND1, MEK-1 Frequency, cDNA microarray validation 2001 (11) Prostate cancer 371 FISH NMYC, MYC, HER2, CCND1 Frequency, tumor progression 1999 (12)



Biomarker barcodes: multiplexed microfluidic ...

Jul 12, 2021·A biomarker barcode of breast cancer patient-derived TMA was quickly scanned by a slide scanner and compared to the conventional method for breast cancer diagnosis. This “barcode-IHC” concept, which has been verified by performing multiplexed microfluidic IHC on CMA and TMA samples, provides high reproducibility and the potential of high ...

BRCA1 protein expression and subcellular localization in ...

Sep 01, 2017·BRCA1 IHC staining was lower in breast cancer cases than normal breast tissue in both the cytoplasmic (cancer: 39.2±1.9; Normal: 58.5±3.9, p = 0.037) and nuclear fractions (cancer: 65.4±2.3; normal: 81.8±4.4, p = 0.007) . BRCA1 IHC staining was very weak in the stromal compartment in both normal breast and breast cancer tissues.

BRCA1 protein expression and subcellular localization in ...

Sep 01, 2017·BRCA1 IHC staining was lower in breast cancer cases than normal breast tissue in both the cytoplasmic (cancer: 39.2±1.9; Normal: 58.5±3.9, p = 0.037) and nuclear fractions (cancer: 65.4±2.3; normal: 81.8±4.4, p = 0.007) . BRCA1 IHC staining was very weak in the stromal compartment in both normal breast and breast cancer tissues.

Human Paraffin Embedded Tissue Array - US Biomax

Breast cancer with adjacent normal breast tissue array, including pathology grade, TNM and clinical stage, with IHC results ER/PR/Her2, 110 cases/110 cores, replacing BC081120e 110 $255.00

Tissue microarrays for comparing molecular features with ...

Dec 05, 2005·Breast cancer tissue microarray. A total of 2,222 formalin‐fixed (buffered neutral aqueous 4% solution), paraffin‐embedded tumors were available in the Institute of Pathology, University Hospital Basel, the Institute for Clinical Pathology in Basel and the Triemli Hospital in Zürich.

Reliability of a computational platform as a surrogate for ...

Background. Pathologist and computational assessments have been used to evaluate immunohistochemistry (IHC) in epidemiologic studies. We compared Definiens Tissue Studio® to pathologist scores for 17 markers measured in breast tumor tissue microarrays (TMAs) [AR, CD20, CD4, CD8, CD163, EPRS, ER, FASN, H3K27, IGF1R, IR, Ki67, phospho-mTOR, PR, PTEN, RXR, …

Immunohistochemistry Microarrays - PubMed

Immunohistochemistry (IHC) on tissue sections is widely used for quantifying the expression patterns of proteins and is part of the standard of care for cancer …

Cancer Human Cell Line Microarrays - Bio SB

The 11-Core Breast Cancer Cell Line Microarray consist of 11- 2 mm cores of formalin-fixed paraffin-embedded cell lines which were assembled in array fashion to allow multiplex molecular pathology analysis and validation of reagents, or to be used as tissue controls for Immunohistochemistry and/or in situ hybridization (CISH and FISH) applications.

(PDF) Tissue microarrays for comparing molecular features ...

In Material and methods summary, all expected associations between Ki67 and the analyzed molecular markers could be reproduced with high statistical signif- Breast cancer tissue microarray icance using a TMA containing only one tissue sample per tumor, A total of 2,222 formalin-fixed (buffered neutral aqueous 4% measuring 0.6 mm in diameter.

Breast Cancer 100 Tissue Microarray

Breast Cancer 100 TMA ~100 samples from ~100 donors. Molecular data available for this array are ER, PR, HER2 (IHC), Ki67. Cores are 1.00 MM.

US Biomax - Tissue Array and Tissue Microarray of 1684 ...

Click Core Images Tab in the specification sheet page to view the scanned images up to 400x amplification through FullScanViewer provided by US Biomax. For example, 616 core high-density array or 208 core high-density array and 96 core array. News: Cancer tissue arrays with follow-up data are available. They are Prostate , colon, breast, lung ...

Tissue microarray technology in breast cancer HER2 ...

Mar 28, 2007·Tissue microarrays (TMAs) as current medical research tools significantly lower the costs of immunohistochemical examinations (IHC) and fluorescence in situ …

Digital Image Analysis of Ki-67 Stained Tissue Microarrays ...

Jul 20, 2020·We assessed DIA-Ki-67 score on tissue microarrays (TMAs) from breast cancer patients in a case–control study including 541 ER-positive and 300 ER-negative recurrent cases and their non-recurrent controls, matched on ER-status, cancer stage, menopausal status, year of diagnosis, and county of residence.

Comparing computer-generated and pathologist-generated ...

Sep 08, 2015·Tissue microarrays (TMAs) have become a valuable resource for biomarker expression in translational research. Immunohistochemical (IHC) …

Immunohistochemistry: Current Applications in Breast Cancer

Immunohistochemistry (IHC) is used to characterize intracellular proteins or various cell surfaces in all tissues. Individual markers or more often panels of various marker proteins can be used to characterize various tumor subtypes, confirm tissue of origin, distinguish metastatic from primary tumor and provide additional information which may be important for prognosis, predicting response ...

Biomolecules | Free Full-Text | Lumican in Carcinogenesis ...

Furthermore, when the tissue microarrays and tissue sections were analyzed, lumican was found to be expressed by both transformed cells and the stroma of colon adenomas and carcinomas. Notably, it was more frequently detected in carcinoma than adenoma cells and in carcinomas and high-risk adenomas combined compared withlow-risk adenomas [ 44 ].

Breast Cancer 100 Tissue Microarray

Breast Cancer 100 TMA ~100 samples from ~100 donors. Molecular data available for this array are ER, PR, HER2 (IHC), Ki67. Cores are 1.00 MM.

Tissue Microarray (TMA) - Cancer and Normal Arrays | OriGene

OriGene Tissue Microarrays (TMAs) are comprised of human cancer and normal tissues and suitable for high-throughput molecular IHC analysis. These FFPE TMA slides are created from high-quality tissue samples, and include datasheets with pathologist-verified diagnosis, tissue, grade and TNM staging. Extensive QC process.

(PDF) Tissue Microarray (TMA) Versus Whole Section ...

Tissue Microarray (TMA) Versus Whole Section Immunohistochemistry in the Assessment of ER/PR and Her-2/neu Status in a Breast Cancer Series from Sudan May 2013 The Breast Journal 19(4)

PM2a-ER Breast cancer with ER Immunohistochemistry control ...

Microarray Panel: Breast carcinoma with Estrogen Receptor (ER) IHC bio-marker control tissue microarray, containing 10 invasive breast carcinoma (with …

Algorithms to Assess Hormonal Status From Tissue ...

Jul 26, 2019·Findings In this diagnostic study of tissue microarray hematoxylin-eosin–stained images from 5356 patients with breast cancer, molecular biomarker expression was found to be significantly associated with tissue histomorphology. A deep learning model was able to predict estrogen receptor expression solely from hematoxylin-eosin–stained ...

Cancer Human Cell Line Microarrays - Bio SB

The 11-Core Breast Cancer Cell Line Microarray consist of 11- 2 mm cores of formalin-fixed paraffin-embedded cell lines which were assembled in array fashion to allow multiplex molecular pathology analysis and validation of reagents, or to be used as tissue controls for Immunohistochemistry and/or in situ hybridization (CISH and FISH) applications.

Storage conditions and immunoreactivity of breast cancer ...

Introduction. Immunohistochemistry (IHC) is a well-established method for the detection of protein expression in human tissues. It is quick, cost effective, and …

Frequency of breast cancer subtypes among African American ...

Feb 06, 2018·We quantified the expression of six tumor biomarkers using automated methods for scoring IHC staining of tissue microarrays (TMAs) comprising 1381 cases of invasive breast cancer in African American (AA) women from the African American Breast Cancer Epidemiology and Risk (AMBER) consortium . The aim of this study was to optimize IHC-based tumor ...

Assessment of Automated Image Analysis of Breast Cancer ...

Keywords: Tissue Microarray, Immunohistochemistry, Automated Scoring, Breast Cancer, Reproducibility Introduction There is increasing evidence that risk factor …

Human Breast Tissue MicroArray (Cancer) (NBP2-30212 ...

Human Breast Tissue Microarray (Cancer)/ human tissue microarray containing 40 breast cancer tumor tissues and 9 paired normal breast tissues was used for …