types of explants in tissue culture model

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Effect of Cultivar and Explants Type on Tissue Culture ...- types of explants in tissue culture model ,Effect of Cultivar and Explants Type on Tissue Culture Regeneration of Three Nigerian Cultivars of TomatoesExplants - an overview | ScienceDirect TopicsExplants are prepared by dissecting and mincing placental tissue into small masses, followed by plating into appropriate tissue culture wells and incubation for a finite period of time (Miller et al., 2005; Aplin, 2006). Due to the nature of their preparation, explants are essentially a heterogeneous population of placental cells with ...



Nucleus pulposus explant culture model.

Nucleus pulposus explant culture model. Feng G (1), Wan Y, Shen FH, Li X. (1)Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA. Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are ...

Tissue Culture Models

Mar 25, 2009·Types of Tissue Culture. Tissue culture is a commonly used generic term for the in vitro cultivation of cells, attributed to the early cultures that generally consisted of heterogeneous cultures of crudely disaggregated tissues. Currently, many terms are used that can be encompassed by the term: organ culture, cell culture, primary explants, and ex vivo propagation all concern the in vitro ...

Plant tissue culture media and practices: an overview ...

Apr 15, 2019·Plant tissue culture systems allow for the rearing of whole plants, organs, tissues, or cells under controlled aseptic conditions in the laboratory. The tissue culture system supplies all nutrients, energy, and water necessary for plant or explant growth through the basal medium.

Plant tissue culture - SlideShare

Jan 24, 2017·PLANT TISSUE CULTURE • Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation . 4.

Application of human mucosal explants culture in the HIV-1 ...

Human fetal intestinal explants (14-21 weeks) were established in culture and exposed to HIV-1. Tissue culture fluid was assayed for p24 antigen and reverse transcriptase activity over a 14-day ...

The culture of gut explants: A model to study the mucosal ...

Nov 01, 2016·Jarry et al. (2008, 2015) with use of an ex vivo model of mucosa explant cultures confirmed the correlation between secreted IFN-γ/IL-18 levels, and caspase-1 activation, in intestinal CD mucosa explants. In the modern era, the culture of intestinal explants followed by an …

Explants Research Papers - Academia.edu

The effect of different concentrations of BA and Kin (0, 0.5, 1 and 2 mg/L) in combination with 0.1 mg/L NAA on regeneration of different twin scale type of amaryllis was evaluated. Explants were divided as twin scales and classified in 4 groups, so that the outermost twin scales was grouped in …

Human retinal model systems: Strengths, weaknesses, and ...

Sep 13, 2021·Retinal explants are also a poor model for studying development of ganglion cells as they begin to die after only one week in culture (Osborne et al., 2016). If working from a fetal retina, tissue growth is not as rapid as dissociated cells or in vivo growth, therefore the number of …

Frontiers | Tumor Tissue Explant Culture of Patient ...

Signaling pathways can be targeted in ex vivo 2 × 2 × 2 mm 3 tissue explants culture. (A) Schematic diagram of the workflow. 2 × 2 × 2 mm 3 tissue explants from the xenograft tumor of SE-Pt#1 were cultured with EGFR inhibitor, erlotinib, PI3Kα inhibitor, BYL719 and chemotherapeutic drug, cisplatin for 24 h. Western blotting and ...

Types of Explants used in Tissue Culture

Types of Explants used in Tissue Culture. All parts of plants can be used as explants for tissue culture purposes: leaves, stems, a portion of shoots, flowers, anthers, ovary, single undifferentiated cells, or mature tissues. However, the cells should be capable of de …

Human skin explant model for the investigation of topical ...

Dec 03, 2020·The most abundant extra-cellular matrix component is type I ... Human tissue explants were cultured ... W. et al. Application of a partial-thickness human ex vivo skin culture model in ...

What Are The Types Of Explants Used In Plant Tissue ...

Tag - What Are The Types Of Explants Used In Plant Tissue Culture. ... Plant Tissue Culture Questions and Answers: In the following write-up let us talk about " Common Plant Tissue Culture Questions and Answers" What is Plant Tissue... Agriculture Farming. Vanilla …

The evolution and use of skin explants: Potential and ...

The design of our test system was inspired by Lebonvallet and colleagues [27] who described a "Trowell-type organ culture chamber" for skin explants, that mimics a physiological environment and ...

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in ...

Nov 03, 2019·Tissue culturing Transfer the explants to a sterile 96-well plate in a laminar flow hood. Wash the explants 3 times in culture medium or PBS and culture them in 200 μL of culture medium per well until the start of the experiment. Use a washout period of 1 day to synchronize biopsy cellular activity and passive biomarker release.

Explant - an overview | ScienceDirect Topics

Explants are prepared by dissecting and mincing placental tissue into small masses, followed by plating into appropriate tissue culture wells and incubation for a finite period (Miller et al., 2005). Because of the nature of their preparation, explants are essentially a heterogeneous population of …

What are Explants? - Science Samhita

Jan 27, 2018·Explants are small pieces of plant parts or tissues that are aseptically cut and used to initiate a culture in a nutrient medium. Explants can be taken from different parts of a plant such as shoots, leaves, stems, flowers, roots, and from many types of mature cells provided they are able to …

Long-term culture of human esophageal explants and cells

Human esophageal epithelium obtained from intermediate autopsies (less than 12 h) was maintained as cell and explant cultures. In order to develop a serum-free, defined media culture model, several medias and additives were evaluated. The viability and differentiation of the epithelial cells culture …

Chapter 3 plant tissue culture - SlideShare

Oct 02, 2018·STATIC CULTURE (CALLUS CULTURE) Callus – a mass of undifferentiated plant cells grown on solid media from plant part/explant. It may initiate from explants of any multi-cellular plant. The organs such as root, stem tips, leaves, flowers and fruit are grown on solid media. The cell groups are initiated from: - Explant/Segments of root, stem or ...

Long-term culture of human esophageal explants and cells

Human esophageal epithelium obtained from intermediate autopsies (less than 12 h) was maintained as cell and explant cultures. In order to develop a serum-free, defined …

Explant Culture - an overview | ScienceDirect Topics

Explant culture is a technique used for culturing tissue pieces themselves. We have applied this technique to the primary culture of human respiratory epithelium and observed that in the cell outgrowth around the explant, well-differentiated cells are present as well as dedifferentiated migrating cells at the periphery of the outgrowth (Chevillard et al., 1991; Plotkowski et al., 1991).

Frontiers | A Novel 3D Skin Explant Model to Study ...

Sep 14, 2017·Our results demonstrate that D. nodosus can invade the skin explant, and that altered expression of key inflammatory markers could be quantified in the culture media. The viability of explants was assessed by tissue integrity (histopathological features) and cell death (DNA fragmentation) over 76 hours showing the model was stable for 28 hours.

A tissue-culture model of cartilage breakdown in ...

May 15, 1979·A tissue-culture model of cartilage breakdown in rheumatoid arthritis. Quantitative aspects of proteoglycan release. Steinberg J, Sledge CB, Noble J, Stirrat CR. 1. The destruction of articular cartilage in human rheumatoid and other arthritides is the result of diverse …

Explant culture of gastrointestinal tissue: a review of ...

Mar 08, 2011·The gastrointestinal (GI) tract is an important target organ for the toxicity of xenobiotics. The toxic effects of xenobiotics on this complex, heterogeneous structure have been difficult to model in vitro and have traditionally been assessed in vivo. The explant culture of GI tissue offers an alternative approach. Historically, the organotypic culture of the GI tract proved far more ...

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in ...

Ex vivo culture systems cover a broad range of experiments dedicated to studying tissue and cellular function in a native setting. Cartilage is a unique tissue …

(PDF) Methods paper: An improved surface sterilization ...

Presterilization of excised explants with Benomyl (0.2%) can improve a cleanness and aliveness of all types of explants, especially when followed through surface sterilization done by mercury ...

Human skin explant model for the investigation of topical ...

Dec 03, 2020·The most abundant extra-cellular matrix component is type I ... Human tissue explants were cultured ... W. et al. Application of a partial-thickness human ex vivo skin culture model in ...